Analysis of the poplar phloem proteome and its response to leaf wounding.
Identifieur interne : 003704 ( Main/Exploration ); précédent : 003703; suivant : 003705Analysis of the poplar phloem proteome and its response to leaf wounding.
Auteurs : Nicole J. Dafoe [Canada] ; Arezoo Zamani ; Abul K M. Ekramoddoullah ; Dustin Lippert ; Jörg Bohlmann ; C Peter ConstabelSource :
- Journal of proteome research [ 1535-3893 ] ; 2009.
Descripteurs français
- KwdFr :
- Banque de gènes (MeSH), Chromatographie en phase liquide (MeSH), Contrainte mécanique (MeSH), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Immunotransfert (MeSH), Microscopie de fluorescence (MeSH), Phloème (génétique), Phloème (métabolisme), Populus (génétique), Populus (métabolisme), Protéines végétales (classification), Protéines végétales (génétique), Protéines végétales (métabolisme), Protéome (classification), Protéome (génétique), Protéome (métabolisme), Protéomique (méthodes), Régulation de l'expression des gènes végétaux (MeSH), Spectrométrie de masse en tandem (MeSH), Électrophorèse bidimensionnelle sur gel (MeSH).
- MESH :
- classification : Protéines végétales, Protéome.
- génétique : Feuilles de plante, Phloème, Populus, Protéines végétales, Protéome.
- métabolisme : Feuilles de plante, Phloème, Populus, Protéines végétales, Protéome.
- méthodes : Protéomique.
- Banque de gènes, Chromatographie en phase liquide, Contrainte mécanique, Immunotransfert, Microscopie de fluorescence, Régulation de l'expression des gènes végétaux, Spectrométrie de masse en tandem, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- Chromatography, Liquid (MeSH), Electrophoresis, Gel, Two-Dimensional (MeSH), Gene Expression Regulation, Plant (MeSH), Gene Library (MeSH), Immunoblotting (MeSH), Microscopy, Fluorescence (MeSH), Phloem (genetics), Phloem (metabolism), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Proteins (classification), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (genetics), Populus (metabolism), Proteome (classification), Proteome (genetics), Proteome (metabolism), Proteomics (methods), Stress, Mechanical (MeSH), Tandem Mass Spectrometry (MeSH).
- MESH :
- chemical , classification : Plant Proteins, Proteome.
- genetics : Phloem, Plant Leaves, Plant Proteins, Populus, Proteome.
- metabolism : Phloem, Plant Leaves, Plant Proteins, Populus, Proteome.
- methods : Proteomics.
- Chromatography, Liquid, Electrophoresis, Gel, Two-Dimensional, Gene Expression Regulation, Plant, Gene Library, Immunoblotting, Microscopy, Fluorescence, Stress, Mechanical, Tandem Mass Spectrometry.
Abstract
Phloem exudate collected from hybrid poplar (Populus trichocarpa x Populus deltoides) was estimated to have more than 100 proteins, of which 48 were identified using LC-MS/MS. Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P<0.05) upregulated 24 h after leaf wounding. These were identified as pop3/SP1 and a thaumatin-like protein. This is the first characterization of a phloem proteome from a tree species.
DOI: 10.1021/pr800968r
PubMed: 19245218
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P<0.05) upregulated 24 h after leaf wounding. These were identified as pop3/SP1 and a thaumatin-like protein. This is the first characterization of a phloem proteome from a tree species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19245218</PMID><DateCompleted><Year>2009</Year><Month>06</Month><Day>23</Day></DateCompleted><DateRevised><Year>2009</Year><Month>05</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1535-3893</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>5</Issue><PubDate><Year>2009</Year><Month>May</Month></PubDate></JournalIssue><Title>Journal of proteome research</Title><ISOAbbreviation>J Proteome Res</ISOAbbreviation></Journal><ArticleTitle>Analysis of the poplar phloem proteome and its response to leaf wounding.</ArticleTitle><Pagination><MedlinePgn>2341-50</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/pr800968r</ELocationID><Abstract><AbstractText>Phloem exudate collected from hybrid poplar (Populus trichocarpa x Populus deltoides) was estimated to have more than 100 proteins, of which 48 were identified using LC-MS/MS. Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P<0.05) upregulated 24 h after leaf wounding. These were identified as pop3/SP1 and a thaumatin-like protein. This is the first characterization of a phloem proteome from a tree species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dafoe</LastName><ForeName>Nicole J</ForeName><Initials>NJ</Initials><AffiliationInfo><Affiliation>Centre for Forest Biology and Department of Biology, University of Victoria, Victoria, BC, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zamani</LastName><ForeName>Arezoo</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Ekramoddoullah</LastName><ForeName>Abul K M</ForeName><Initials>AK</Initials></Author><Author ValidYN="Y"><LastName>Lippert</LastName><ForeName>Dustin</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Bohlmann</LastName><ForeName>Jörg</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Constabel</LastName><ForeName>C 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Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015151" MajorTopicYN="N">Immunoblotting</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052585" MajorTopicYN="N">Phloem</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName 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MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013314" MajorTopicYN="N">Stress, Mechanical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>2</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>2</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>6</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19245218</ArticleId><ArticleId IdType="doi">10.1021/pr800968r</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Bohlmann, Jorg" sort="Bohlmann, Jorg" uniqKey="Bohlmann J" first="Jörg" last="Bohlmann">Jörg Bohlmann</name><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name><name sortKey="Ekramoddoullah, Abul K M" sort="Ekramoddoullah, Abul K M" uniqKey="Ekramoddoullah A" first="Abul K M" last="Ekramoddoullah">Abul K M. Ekramoddoullah</name><name sortKey="Lippert, Dustin" sort="Lippert, Dustin" uniqKey="Lippert D" first="Dustin" last="Lippert">Dustin Lippert</name><name sortKey="Zamani, Arezoo" sort="Zamani, Arezoo" uniqKey="Zamani A" first="Arezoo" last="Zamani">Arezoo Zamani</name></noCountry><country name="Canada"><noRegion><name sortKey="Dafoe, Nicole J" sort="Dafoe, Nicole J" uniqKey="Dafoe N" first="Nicole J" last="Dafoe">Nicole J. Dafoe</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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